Trypanosoma cruzi infection of the placenta in women with chronic chagas disease: direct molecular characterization and tissue tropism of natural populations

BISIO M; BURGOS JM; SEIDENSTEIN ME; DUFFY T; PONTORIERO R; LEVIN MJ; MACCHI L; SCHIJMAN AG

Los Cocos, Córdoba, Argentina

Simposio; III Latin-American Symposium on Maternal-Fetal Interaction and Placenta: Basic & Clinical Research; 2007

Methods: Placental specimens collected from 49 women with chronic Chagas disease were analysed for detection of T. cruzi infection: 43 by PCR targeted to minicircle sequences (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. were analysed for detection of T. cruzi infection: 43 by PCR targeted to minicircle sequences (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. Placental specimens collected from 49 women with chronic Chagas disease were analysed for detection of T. cruzi infection: 43 by PCR targeted to minicircle sequences (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. T. cruzi infection: 43 by PCR targeted to minicircle sequences (kDNA) and 6 by microscopical observation (MO). Molecular typing of parasite lineages was performed by lineage-specific PCR analysis. In addition, satellite DNA based quantification of T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. T. cruzi load was performed by Real-time (qPCR). In addition, to estimate the degree of parasitemia during pregnancy, 31 of them from which at least two consecutive blood samples were analysed by kDNA-PCR were classified in two different profiles: high (only positive findings) and low (with negative findings) parasitemia. Results: We detected T cruzi infection in 14 (28.6%) placental specimens. The sensitivity was 30.2% (13/43) by PCR and 16.7% by MO (1/6). The qPCR was useful to quantify placental parasitic load in two specimens from one placenta that showed 104 and 116 parasites/107 host cells. Based on PCR analysis, we classified those parasites belonged to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. was 30.2% (13/43) by PCR and 16.7% by MO (1/6). The qPCR was useful to quantify placental parasitic load in two specimens from one placenta that showed 104 and 116 parasites/107 host cells. Based on PCR analysis, we classified those parasites belonged to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. We detected T cruzi infection in 14 (28.6%) placental specimens. The sensitivity was 30.2% (13/43) by PCR and 16.7% by MO (1/6). The qPCR was useful to quantify placental parasitic load in two specimens from one placenta that showed 104 and 116 parasites/107 host cells. Based on PCR analysis, we classified those parasites belonged to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. 7 host cells. Based on PCR analysis, we classified those parasites belonged to lineage IId. Fourteen out of the 31 women followed up during pregnancy had high parasitemia (4 of them with positive findings in placenta: 28.6) and 17 with low parasitemia (8 of them with positive placenta samples, 47.1%). Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. Conclusions: kDNA PCR was more sensitive than MO to detect T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism. T. cruzi placental infection. Our results indicate that placental parasitism is not necessarily related to high bloodstream parasitemia. Moreover, women with PCR negative blood but PCR positive placenta specimens could be infected by parasitic strains with placental tropism.