INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
"Caracterización de un homólogo del gen wee1 en Tripanosomátidos
Autor/es:
BOYNAK, N.Y., ROJAS, F. TÉLLEZ-IÑÓN M. T.
Lugar:
Mar del Plata RA
Reunión:
Congreso; XLIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology (SAIB); 2007
Institución organizadora:
Argentine Society for Biochemistry and Molecular Biology (SAIB)
Resumen:
In eukaryotic cells activation of the complex cyclin B- cdc2 kinase is a pivotal step in mitotic initiation of cell cycle. Phosphorylation of cdc2 in the Tyr15 inhibitory site by the Wee1 kinase suppresses the cdc2 activity during interphase. In several organisms homologues of Wee have been identified. In Trypanosomatids, the presence of kinases that modify CRKs (Cyclin- Related Kinases) have not been reported. Querying the Trypanosoma genome database (GeneDB), we have identified a homologue of the Wee1 family. T cruzi Wee sequence has 1761 bp, which encodes a protein of 586 aa with a predicted molecular weight of 65 kDa. In T brucei, the sequence identified contains 1812 bp and a protein of 603 aa (66.5 kDa). The T. cruzi and T. brucei wee homologues are 52 % identical to one another, and only 20-30 % identical to A. thaliana, S. pombe and human homologues. The protein kinase domain exhibits the greatest similarity at the amino acid level. The Wee1 T. brucei homologue was cloned into the expression vector pDEST17 with a N-terminal His tag. Polyclonal antibodies raised in mouse against His-TbWee1 detected the recombinant Wee protein and a protein of the expected weight in T. cruzi and T. brucei extracts. Reverse genetics is being carried out to identify the function that this kinase has in the cell cycle of these eukaryotic cells.