Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro

MARÍA E. SEGRETIN, EZEQUIEL M. LENTZ, SONIA A. WIRTH, MAURO M. MORGENFELD AND FERNANDO F. BRAVO-ALMONACID

Congreso; Congreso Internacional Biotecnología Habana 2011: Agro-Biotecnología, contribuyendo a enfrentar los retos globales.; 2011

CIGB

Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene and the selective gene aadA has been published. We successfully expressed β-glucuronidase in transplastomic Solanum tuberosum (var. Desiree) plants, with accumulation levels for the recombinant protein of up to 41% of total soluble protein in mature leaves. To our knowledge, this is the highest expression level reported for a heterologous protein in S. tuberosum. Accumulation of the recombinant protein in soil-grown minitubers was very low, as described in previous reports. Interestingly, microtubers developed in vitro showed higher accumulation of β-glucuronidase. As light exposure during their development could be the trigger for this high accumulation, we analyzed the effect of light on β-glucuronidase accumulation in transplastomic tubers. Exposure to light during 8 days increased β-glucuronidase accumulation in soil-grown tubers, acting as a light-inducible expression system for recombinant protein accumulation in tuber plastids. In summary, we show that plastid transformation in potato allows the highest recombinant protein accumulation in foliar tissue described so far for this food crop. We also demonstrate that in tubers high accumulation is possible and depends on light exposure. Because tubers have many advantages as protein storage organs, these results could lead to new recombinant protein production schemes based on potato.