Localization and Enzimatic Activity of Aurora Kinases from Trypanosoma cruzi

FASSOLARI, MATÍAS; MEYER, CRISTIAN G.; TORRES, HECTOR N.; FLAWIÁ, MIRTHA M.; ALONSO, GUILLERMO D.

Puerto Madryn

Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

In T. cruzi we’ve already identified three genes that codify for aurora kinases (TcAUK1, 2 and 3) and have described their genomic organization and expression profile at mRNA level. We’ve also established TcAUK1 as a nuclear protein in epimastigotes that its expression seems to be regulated in a cell cycle-dependent way. In this work we extend the study of cellular localization to the others TcAUKs, expressing them as fusion proteins with GFP in epimastigotes of T. cruzi. As part of the biochemical characterization of these enzymes we’ve performed kinase assays, using recombinant TcAUKs expressed in E. coli. With these assays we could established that TcAUKs show greater activity levels when Mn+2 is used as cofactor.