INGEBI 02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
10-23 DNAzyme modified with (2R) and (2S) 2-deoxy-2-C-methyluridine in the catalytic core
Autor/es:
LAURA ROBALDO, JAVIER M. MONTSERRAT, ADOLFO M. IRIBARREN
Revista:
BIOORGANIC & MEDICINAL CHEMISTRY.
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 vol. 20 p. 4367 - 4370
ISSN:
0968-0896
Resumen:
The catalytic core of a 10-23 DNAzyme was modified using (2´R), (2´S)-2´-deoxy-2´-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg2+ concentrations, indicating that certain 2´-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2´-C-methyl-2´-deoxynucleosides increased the stability
