INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy
Autor/es:
HELENA CERETTI, BELÉN PONCE, SILVANA RAMÍREZ,JAVIER M. MONTSERRAT
Revista:
ELECTROANALYSIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Año: 2010 vol. 22 p. 147 - 150
ISSN:
1040-0397
Resumen:
This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 - 55 mM), finding a consequent variation of molecular surface density (3.5 1011 - 2.8 1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 mM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorotioate anchoring thermal stability was compared to thiol immobilization.