INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
Identification of core components of the Exon Junction Complex in Trypanosomes
Autor/es:
BERCOVICH, NATALIA; LEVIN, MARIANO; CLAYTON, CHRISTINE; VAZQUEZ, MARTIN
Revista:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2009 vol. 166 p. 190 - 193
ISSN:
0166-6851
Resumen:
In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20–24 nt upstream of the exon–exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5′ trans-splicing of a 39-nt RNA sequence. Here we show that trypanosomes have a conserved Mago and a divergent Y14 protein, but we were unable to identify a Btz orthologue. We demonstrate that Mago and Y14 form a stable heterodimer using yeast two hybrid analyses. We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport.