INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
Anti-beta1-adrenergic receptor autoantibodies in patients with chronic Chagas heart disease
Autor/es:
LABOVSKY V.; SMULSKI C.R.; GOMEZ K.; LEVY G.V.; LEVIN M.J.
Revista:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Editorial:
Blackwell Publishing
Referencias:
Año: 2007 vol. 148 p. 440 - 449
ISSN:
0009-9104
Resumen:
Chronic Chagas heart disease (cChHD), a chronic manifestation of the Trypanosoma cruzi infection, is characterized by high antibody (Ab) levels against C-terminal region of the ribosomal P proteins (i.e. peptide R13, EEEDDDMGFGLFD) which bears similarity with the second extracellular loop of b1-adrenergic receptor (b1-AR, peptide H26R HWWRAESDEARRCYNDPKCCDFVTNR). Since it has not been clearly demonstrated that IgGs from cChHD patients bind to native human b1-AR, the aim of this study was to investigate further the physical interaction between cChHD IgGs and the human b1-AR. Immunofluorescence assays demonstrated the binding of these Abs to the receptor expressed on stably transfected cells, together with a b1-AR agonist like effect. In addition, immunoadsorption of the serum samples from cChHD patients with a commercially available matrix containing peptides representing the first and the second extracellular loop of the b1-AR, completely abolished the reactivity against H26R peptide and the physiological response to the receptor. The follow up of this specificity after in vitro immunoadsorption procedures suggests that this treatment might be used to significantly diminish the serum levels of anti-b1-AR Abs in patients with Chagas heart disease.