INGEBI 02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds
Autor/es:
MEDICI, ROSARIO; GARAYCOECHEA, JUAN; VALINO, ANA; PEREIRA, CLAUDIO; LEWKOWICZ, ELIZABETH; IRIBARREN. ADOLFO
Revista:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2014 vol. 98 p. 3013 - 3022
ISSN:
0175-7598
Resumen:
Natural and modified nucleoside-5′-monophosphates and their precursors are valuable compounds widely used in biochemical studies. Bacterialnonspecific acid phosphatases (NSAPs) are a group of enzymes involved in the hydrolysis of phosphoester bonds, and some of them exhibit phosphotransferase activity. NSAP containing Enterobacter aerogenes andRaoultella planticolawhole cells were evaluated in the phosphorylation of a wide range of nucleosides and nucleoside precursors using pyrophosphate as phosphate donor. To increase the productivity of the process, we developed two genetically modified strains ofEscherichia coliwhich overexpressed NSAPs ofE. aerogenesandR. planticola. These new recombinant microorganisms (E. coliBL21 pET22b-phoEa andE. coli BL21 pET22b-phoRp) showed higher activity than the corresponding wild-type strains. Reductions in the reaction times from 21hto60min,from4hto15min,andfrom24hto40minin cases of dihydroxyacetone, inosine, and fludarabine, respectively, were obtained.
