INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol
Autor/es:
DE VAS, M.; PORTAL, P.; ALONSO, G.; SCHLESINGER, M.; FLAWIÁ, M.M.; TORRES, H.N.; FERNÁNDEZ VILLAMIL, S.; PAVETO, CRISTINA
Revista:
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: San Francisco; Año: 2010 vol. 41 p. 99 - 108
ISSN:
0020-7519
Resumen:
Trypanosoma cruzi flavoproteins TcCPR-A, TcCPR-B and TcCPR-C are members of the NADPH-dependent cytochrome P-450 reductase family expressed in the parasite. Epimastigotes overexpressing TcCPR-B and TcCPR-C showed enhanced ergosterol biosynthesis and increased NADP+/NADPH ratio. Transgenic parasites with augmented ergosterol content presented a higher membrane order with a corresponding diminished bulk-phase endocytosis. These results support a significant role for TcCPR-B and TcCPR-C in the sterol biosynthetic pathway and for the first time reveals the participation of more than one CPR in this metabolic route. Notably, TcCPR-B was found in reservosomes while TcCPR-C localized in the endoplasmic reticulum. In addition, we suggest a different role for TcCPR-A, since its overexpression is lethal, displaying cells with an increased DNA content, aberrant morphology and severe ultrastructural alterations.