Resveratrol effect on renal osmoprotection: modulation of cox-2 expression

PARRA, LEANDRO; FERNÁNDEZ TOME, MARÍA C.; ERJAVEC, LUCIANA C.; CASALI, CECILIA

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

SAIC, SAFIS y SAI

Resveratrol (RSV) is a polyphenol present in several plants. Nowadays it is sold as an over-the-counter dietary supplement mainly due to its antioxidant, anti-inflammatory and antitumoral effects. Animalrenal injury models describe RSV benefícial effects, while studies with chronic intake of RSV demonstrate nephrotoxic effects. Hence, RSV effects on renal tissue are still controversial. Due to the urinaryconcentrating mechanism, renal medullary interstitium presents an elevated osmolality that can abruptly vary depending on the hydric state of the body, reaching values up to 800-1200 mOsm/kg H2O. To survive in this environment, renal cells activate protective pathways. We demonstrated that renal epithelial cells (MDCK) subjected to high osmolarity undergo an adaptive process during the first 24h, in which the transcription of the osmoprotective gene cyclooxygenase 2 (COX-2) is activated, among others. After 48h, these cells are already adapted and acquire a polarized epithelium morphology. In the present work, we evaluate the effect of RSV on adaption and differentiation mechanisms, focusing particularly on COX-2 role. To do this, MDCK cells were pretreated with different concentrations of RSV (1, 5, 10, 25μM) and then cultured in hyperosmotic medium (NaCl 125mM, 512 mOsm/kg H2O) for 24 and 48h. Cells were then harvested to obtain cell number and viability. Immunofluorescence (IF), western blot and RT-PCR analysis were performed. RSV significantly decreased cell number in a concentration-dependent manner at 24 and 48h (p