Effects of anthracene and magnetite nanoparticles coated with oleic acid on breast cancer cell proliferation.

MARDIROSIAN, M.N.; NUÑEZ, M; HIELPOS, M.S.; VENTURINO A; LASAGNA, M.; COCCA C.

Buenos Aires

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

The demand for crude oil hydrocarbons represents a growing concern worldwide due to pollution problems from drilling, production and transportation. Thus, it is important to perform toxicity studies to assess the risk associated with these incidents, not only for the environment but also for humans. Our aim was to evaluate the effects of anthracene, a polycyclic aromatic hydrocarbon (PAH), and magnetite nanoparticles coated with oleic acid (NP) on breast cancer cells proliferation. These NP were developed in the CITAAC for the remediation of water contaminated with PAH.We have previously studied the effects of the water-soluble fraction of petroleum (WSF) simulating a possible oil spill. WSF exposure significantly decreased the viability and significantly increased catalase catalase (CAT) activity of MCF-7 and MDA-MB-231 breast cancer cells compared to controls. As the WSF is a mixture of hydrocarbons we considered important to study the effects of PAHs individually to understand if the observed effects were induced by the individualaction of its components or by the interaction between them. MCF-7 and MDA-MB-231 cell lines were treated for 7d with either anthracene (0; 3,5; 7; 15 and 28 μM) or NP (0; 12,5; 25; 50; 100 and200 mg/L) to perform clonogenic assays. No significant differences were observed in the clonogenicity of anthracene treated cells compared to control. However, the exposure to NP from 12,5 to 200 mg/L and from 25 to 200 mg/L significantly decreased the clonogenicity of MCF-7 and MDA-MB-231 cells respectively (p