IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LINE-1 retrotransposon activation in non-tumorigenic mammary epithelial cells exposed to pesticides. A link to why is breast cancer on the rise?
Autor/es:
ZAPPIA C. DANIEL ; LEGUIZAMÓN M. AGUSTINA; MONCZOR FEDERICO ; PONTILLO CAROLINA ; LASAGNA MARIANELA ; RANDI ANDREA ; MIRET NOELIA V; ZÁRATE LORENA V; COCCA CLAUDIA
Lugar:
Modalidad Virtual
Reunión:
Otro; Reunión Anual de Sociedades de Biociencias - SAIC- SAI - SAFIS; 2020
Institución organizadora:
SAIC
Resumen:
Pesticides exposure is linked to the rise in breast cancer incidence. Long interspersed nuclear element-1 (LINE-1) is inhibited in somatic tissues but its reactivation was implicated in mammary tumorigenesis. The pesticides hexachlorobenzene (HCB) and chlorpyrifos (CPF), both ligands of the aryl hydrocarbon receptor (AhR), increase LINE-1 activation in breast cancer cells MDA-MB-231, through AhR/c-Src and TGF-β1/Smad3 signaling. Our aim was to evaluate if LINE-1 can be activated by pesticide exposure in non-tumorigenic mammary epithelial cells NMuMG. We used environmentally relevant doses of CPF (0.05-50 µM) and HCB (0.005-5 µM).LINE-1 mRNA levels were examined by RT-qPCR, finding an increment after 48 h of exposure with CPF (0.05-5 µM, p