Transferrin transport through the endosomal-exosomal pathway in oligodendrolioma cell line OLN-93 ?

MATTERA, VANESA S; PASQUINI, JM; CORREALE, J; PEREYRA GERBER, P

Cordoba

Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018

Sociedad argentina de neurociencias (SAN)

Transferrin transport through the endosomal-exosomal pathway in oligodendrolioma cell line OLN-93Mattera V.S., Pereyra Gerber P, Correale J. and Pasquini J.M.Studies by our group have shown that apoTransferrin (aTF) has maturational effects on oligodendroglial precursor cells, which allows its use as a potential therapeutic agent in Central Nervous System (CNS) demyelinating diseases. Exosomes are nanoparticles of 20-200 nm secreted by cells which allow intercellular communication through long distances. In this context, the aim of our work is to analyze the effects of intranasally administered exosomes as Tf nanocarriers in a demyelination model. Given that some exosomes contain the Tf receptor (TfR), our interest is to find an easy and quick pathway of intracellular loading of aTf through its binding to the receptor. Oligodendroglioma cells OLN-93 were incubated for 30 min in the presence of human aTf, washed and subsequently incubated for 24 h in DMEMF12 without FCS. Western blot analyses were used to characterize the isolated exosomes with different exosome markers and revealed the presence of the Tf-TfR complex. These results were corroborated using a special type of beads coated by exosomes to be detected by flow cytometry. Coated beads were treated with an anti-CD63 exosome marker and a fluorescent anti-Tf marker.