Participation of TGFbeta on the remyelination process

ANA M. ADAMO.; DEBORA RODRIGUEZ; LAURA GÓMEZ PINTO; PATRICIA MATHIEU

Córdoba

Congreso; XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2019

Sociedad Argentina de Investigación en Neurociencias

Our previous results have demonstrated the interplay between Notch and TGFβ signaling pathways in adult neural progenitor cells (NPC) cultures from subventricular zone (SVZ). In these cultures, TGFβ favored oligodendroglial cell fate and oligodendroglial precursor cell (OPC) proliferation, and induced OPC differentiation into mature OL. Considering the possible participation of TGFβ in the repair mechanisms during demyelination, the aim of the present work is to study, both in vitro and in vivo, the changes induced by this cytokine on the OPC maturation and on the inflammatory process. To analyze TGFβ effect on OPC maturation, in vitro experiments were carried out on OPC primary cultures obtained from newborn rat cerebral cortex. After OPCs treatment with TGFβ for 3 days, no changes in the percentage of PDGFRα+ and MBP+ cells were observed compared to control. However, the presence of TGFβ induced an increase in the morphological complexity of OPC and mature OL. For in vivo experiments control and 14-day-CPZ-treated rats were intraperitoneally injected with TGFβ or its vehicle during 3 days before removing the toxic from the diet. Preliminary results obtained from corpus callosum immunohistochemistry analyses showed a slight increase in MAG+ cells concomitantly with a decrease in Iba1/CD68+ cells in CPZ-treated animals. These results suggest that TGFβ might contribute to OPC differentiation during demyelination and reduce the inflammation associated to demyelination.