IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Iron deficiency via modulation of DMT1 and its effect on CNS physiology in a Drosophila melanogaster model
Autor/es:
MARIO RAFAEL PAGANI; JUANA MARIA PASQUINI; MARÍA SILVINA MARCORA; JORGE CORREALE; AIME BALANZINO
Lugar:
Villa Carlos Paz, Cordoba
Reunión:
Congreso; XXXIV Reunion Anual de la Sociedad Argentina de Neurociencias; 2019
Resumen:
Iron is essential for cell metabolism and plays a fundamental role in the development of the central nervous system (CNS). The neurological signs of iron deficiency in children include decreased cognitive abilities and behavioral problems which have been associated with hypomyelination (Oski and Honig, 1978; Oskiet al., 1983; Lozoff et al., 2006). Studies from our laboratory employing an in vivo gestational iron deficiency model showed alterations in oligodendrocyte (OLG) maturation and, astrocyte (AST) and microglia reactivity (Rosato-Siri et al., 2018). The divalent metal transporter 1 (DMT1) participates in the intracellular export of iron from endosomal compartments as part of the transferrin-bound iron uptake, and also mediates non-transferrin-bound iron uptake. Little is known about the role of DMT1 in glial cells. Recently, it has been reported that DMT1-mediated iron transport promotes OLG maturation and myelination (Cheli et al., 2018). In turn, Malvolio (Mvl) is the only member of the DMT1 family present in Drosophila melanogaster and has shown capacity to transport iron, as indicated by previous reports of iron deficiency in Mvl mutants (Bettedi et al., 2011). As we have previously demonstrated a relationship between OLG and AST, we decided to explore the relevance of DMT1 in glial cells employing Drosophila melanogaster as an in vivo model and studying behavioral and cellular parameters in Mvl mutants. Results showed reduced locomotor activity and impaired habituation capacity of Mvl mutants in an open field paradigm. Inmunofluorescense assays of Mvl mutant fly brains showed no signs of apoptosis. Finally, preliminary analysis of Mvl downregulation in glial cells using the UAS/Gal4 binary system showed decreased locomotion, suggesting that Mvl may impact glial physiology. Further analyses on Mvl downregulation in specific glial subtypes will help unveil the role of DMT1 in the development and maintenance of the CNS.