IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The pesticide chlorpyrifos induces cell migration and modulates MMPs via MAPKs pathway in breast cancer cell lines
Autor/es:
NOELIA MIRET; CLAUDIA COCCA; MARIANELA LASAGNA; ANDREA RANDI; CLARA VENTURA; MARIEL NÚÑEZ
Lugar:
Mar del Plata
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argetina de Investigaciones Clínicas (SAIC); 2018
Institución organizadora:
Sociedad Argentina de Investigaciones Clínicas (SAIC)
Resumen:
Chlorpyrifos (CPF) is one of the insecticides most used in agriculturein our country. We have previously demonstrated that 0.05 uM CPFinduces cell estrogen receptor mediated-proliferation in MCF-7 estrogendependent cell line. However, the proliferation was inhibitedby Reactive Oxygen Species (ROS) increment induced by 50 uMCPF in MCF-7 and in the estrogen independent MDA MB-231 cells.Moreover, 50 uM CPF was able to promote invasiveness in both celllines. Objective: In this work we analyzed the signals involved inmigration and invasiveness CPF-promoted and the role of the metalloproteasesin this action. Methods: MCF-7 and MDA MB231 cellswere exposed to CPF (0.05-50 uM). Wound assay was performedto study cell migration, zimmography to determine gelatinolase activityand Western Blot for phosphorylation protein levels. Results: InMDA MB-231 cells, CPF induced cell migration (300% over control,p< 0.01) after 24 h of exposure. This action was reverted by p38 inhibitor (SB202190 10uM) and ERK 1/2 inhibitor (PD98059 5uM;p