Environmental concentrations of Chlorpyrifos affects the normal female Cyclicity in rats modifying the histological Characteristics of the uterus.

M LASAGNA; ME RODRIGUEZ GIRAULT ; C COCCA; G CAO; C VENTURA; M NUÑEZ; MR RAMOS NIETO; G ALVAREZ; F CHIAPINI

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argetina de Investigaciones Clínicas (SAIC); 2018

Sociedad Argentina de Investigaciones Clínicas (SAIC)

Previously, we demonstrated that the pesticide chlorpyrifos (CPF)acts as an endocrine disruptor in human mammary adenocarcinomacells MCF-7 and in rat mammary gland.We investigated whetherenvironmental concentrations of CPF induce changes in the uterus,and whether this effect depends on the accumulation of CPF inserum or fat in rats.Female 40-day-old Sprague-Dawley rats wereexposed to CPF 0,01 y 1 mg/Kg/día (CPF 0,01 y CPF 1) or vehicle(C) orally for 100 d. We determined the estrous cycle phasedaily. The rats were euthanized in estrus. The uterus, abdominalfat and blood were removed. Serum and fat CPF-levels were determinedby gaseous chromatography. We performed the anatomopathologicalstudy of the uterus. Immunohistochemical expressionof the proliferating cell nuclear antigen (PCNA) was determined.Fractal dimension (FD) analysis, a measure of the irregularity andcomplexity of the tissue was used.Serum levels of CPF were 0.107+/- 0.10 ng/mL and 0.62+/- 0.22 ng/mL in the animals of CPF 0.01 yCPF 1 groups, respectively and undetectable in group C. In fat wefound a significant accumulation of the pesticide in the CPF 1 group(14.16+/-9.48 ng/gr fat; p