IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TGFβ effect during demyelination and remyelination process
Autor/es:
ANA ADAMO ; LAURA GÓMEZ PINTO; PATRICIA MATHIEU ; DÉBORA RODRÍGUEZ
Reunión:
Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018
Resumen:
Adult neural progenitor cells (aNPCs) from the subventricular zone (SVZ) are capable of oligodendrocyte differentiation and this becomes important during the remyelination process following a demyelinating event. Previously, we demonstrated that TGFβ induce oligodendrocyte precursors cells (OPCs) proliferation due an increase in Notch ligand, Jagged, expression in astrocyte, and also oligodendrocyte maturation by a direct action on the OPCs. Considering this, our goal is to study the effect of TGFβ in a demyelination model. Thus, we performed cultures from the SVZ of control or animals treated for 7 days with the toxic cuprizone (CPZ) to induce demyelination. The aNPC obtained were cultured in presence of TGFβ or its vehicle for 4 days. The analysis by immunocytochemistry showed no changes in Nestin, Nestin/GFAP or GFAP positive populations in any of the experimental groups. Cultures obtained from demyelinated animals showed a higher proportion of OPCs (PDGFRα+ cells), than those obtained from control animals. The presence of TGFβ increased PDGFRα+ cells in control cultures, and showed a slight increase in cultures from demyelinated animals. Furthermore, preliminary results obtained by WB analysis of the CC of animals intracranially injected with TGFβ showed that in control or CPZ treated animals TGFβ produced an increase in MBP concomitantly with a decrease in PDGFRα. These results showed that during the demyelination process the TGFβ might contribute in the OPCs differentiation. More experiments are needed to evaluate the real impact of TGFβ during the demyelination/remyelination process.