Cellular lipids changes during adipose-derived stem cells osteogenic differentiation

SETTON PATRICIA; PARRA LEANDRO; FERNÁNDEZ, MARÍA DEL CARMEN

Parana

Congreso; LIV Reunión de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular- SAIB; 2018

SAIB

Adipose-derived Stem Cells (ASC) constitute a promising tool for many applications in regenerative medicine. In the last years, a large numberof works describing the mechanism underlying ASC differentiation was published but the role of lipid metabolism in this process is still unclear.The aim of this work was to evaluate whether osteogenic differentiation of ASC involves changes on lipids content and biosynthesis. For this,adipose tissue was removed from adult Wistar rats and ASC were isolated and culture in low glucose DMEM through 3 passages. After that,ASC were characterized by measuring their capacity to differentiate into the osteogenic and adipogenic lineages and the expression of CD90.Third passage ASC was cultured and in vitro differentiated or not to an osteoblast phenotype with low glucose DMEM containing 100 nMdexamethasone, 10 mM ȕ-glycerophosphate and 5 µg/ml ascorbic acid 2-phosphate. After 21 days, cellular lipids and phospholipid (PL) specieswere quantified. To evaluate lipid synthesis, cells were incubated in the presence of 0,1 µCi [14C(U)]-glycerol for 3h, and then lipidsbiosynthesis was determined. After treatment, differentiated cells showed a decrease on lipid synthesis and phospholipid content compared tocontrol cells. Total PL content decreased by 54%, while PL species content varied in a different manner, leading to a different PL profile withenrichment in sphingomyelin in differentiated cells membranes. PL, Diacylglycerol (DAG) and Triacylglycerol (TAG) synthesis decreased by90, 87 and 86%, respectively. Results show that ASC osteogenic differentiation is accompanied by changes on lipid synthesis and content