TGF-Β effect during the demyelination and remyelination process

LAURA IVONNE GÓMEZ PINTO; DÉBORA VANESA RODRIGUEZ; ANA M. ADAMO ; PATRICIA MATHIEU

Cordoba

Congreso; Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018

Adult neural progenitor cells (NPCs) from the subventricular zone (SVZ) can differentiate into oligodendrocytes, a key aspect during the remyelination process following a demyelinating event. Previously, we demonstrated that TGFβ induces oligodendrocyte precursors cell (OPCs) proliferation through an increase in Notch ligand Jagged1 expression in astrocytes and oligodendrocyte maturation by a direct action on OPCs. The current work studies the effect of TGFβ during demyelination through in vitro and in vivo experiments. SVZ NPCs obtained from control or 7-day cuprizone (CPZ)-treated rats were cultured in the presence of TGFβ or its vehicle for 4 days. Immunocytochemistry showed no changes in Nestin+, Nestin+/GFAP+ or GFAP+ populations in any of the experimental groups. Cultures obtained from demyelinated animals showed a higher proportion of PDGFRα+ cells than those obtained from control animals. The presence of TGFβ increased the proportion of PDGFRα+ cells in control cultures and showed a slight increase in cultures from demyelinated animals. Furthermore, preliminary results obtained from corpus callosum Western blot analyses of animals intracranially injected TGFβ showed an increase in MBP+ cells concomitantly with a decrease in PDGFRα+ cells both in control and CPZ-treated animals. These results indicate that TGFβ might contribute to OPC differentiation during demyelination. More experiments are needed to evaluate the real impact of TGFβ during the whole demyelination/remyelination process