IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TGF-beta and notch pathway participation in oligodendroglial differentiation of adult neural stem cells from the subventricular zone
Autor/es:
DÉBORA RODRÍGUEZ; LAURA GÓMEZ PINTO; PATRICIA MATHIEU; ANA ADAMO
Lugar:
Buenos Aires
Reunión:
Congreso; 2nd FALAN Congress; 2017
Resumen:
Adult neural stem cells (aNSC) are capable of differentiating into neurons, astrocytes and oligodendrocytes throughout life. Also, Notch and transforming growth factor beta 1 (TGFb) signaling pathways play critical roles in controling cell fate. On these considerations, the aim of our work was to study the effect of this cytokine on glial differentiation of aNSC from the subventricular zone (SVZ) and its interaction with the Notch pathway. Wistar rat SVZ aNSC cultures were plated for 48h and then treated with TGFb during four days. Results showed an increase in the percentage of PDGFRa- and NG2-positive oligodendroglial precursor cells, demonstrating TGFb effects on oligodendroglial cell fate decisions. In addition, extended periods of TGFb treatment produced an increase in the population of more differentiated MBP-positive cells. Most interestingly, TGFb receptor II (TGFRII) was detected mainly in Nestin/GFAP-positive cells. Regarding Notch signaling, the expression of Notch ligand Jagged 1 mRNA and Jagged 1-positive cells increased in the presence of TGFb. Moreover, the inhibition of Notch signaling with a gamma-secretase inhibitor blocked TGFb effects on aNSC. Altogether, these data suggest that TGFb might target Nestin/GFAP-positive cells and increase SVZ aNSC oligodendroglial differentiation, at least partly as a result of Notch signaling activation.