TGF-b AND ITS INTERACTION WITH THE NOTCH SIGNALING PATHWAY ON OLIGODENDROGLIAL DIFFERENTIATION OF ADULT NEURAL PROGENITOR CELLS

ANA ADAMO; DEBORA VANESA RODRIGUEZ; LAURA IVONNE GOMEZ PINTO; PATRICIA MATHIEU

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS 2017; 2017

Neural progenitor cells (NPCs) from the subventricular zone (SVZ)are the source of new neurons, astrocytes and oligodendrocytesin the adult brain. Both Notch and TGF-β signaling pathways havebeen shown to act synergistically in co-cultures of mesenchymalstromal cells and NPCs, inducing NPC differentiation into astrocytesand oligodendrocytes. Taking into account the possible participationof NPC in the response mechanisms upon demyelination, the aimof the present work is to study the changes induced by TGF-β andthe underlying interplay between TGF-β and Notch signalling on cellpopulations arising from adult brain SVZ cultures.Wistar rat adult brain SVZ neurospheres were plated in coatedcoverslips and cultured for 48 h and then treated with TGF-β fordifferent times. Results obtained 6 days after plating show a significantincrease (p