Interleukin 17A (IL-17A) should be involved in changes of antibody specificity

JOSÉ L. APARICIO; LILIA A. RETEGUI; MACARENA A. OTTOBRE SABORIDO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. LXII Reunión Anual de La Sociedad Argentina de Investigación Clínica (SAIC). LIII Reunión Anual de La Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB) LXV. Reunión Anual de La Socie; 2017

Introduction: Lactate dehydrogenase elevating virus (LDV) is a persistent and non pathogenic RNA arterivirus that induces NK, macrophages and B- cell activation in mice. It was found that LDV-infection modified Ab specificity to different antigens. This effect was correlated with the release of various cytokines after viral infection.Objectives: The purpose of this work was to explore the role of interleukin 17A (IL-17A) on the changes of Ab specificities. Methods: C57BL/6 mice were inoculated subcutaneously with 25 μg of OVA emulsified in phosphate-buffered saline (PBS) and Complete Freund?s Adjuvant. At day 15, the mice were boosted with the Ag in Incomplete Freund?s Adjuvant. At days 4, 7 and 11, half of the animals were inoculated intraperitoneally with 150 µg of anti-IL-17A MAb (MM17F3) in 200 µl of PBS. Another mouse group were treated as before but infected with 2×107 50% infectious doses of LDV in saline at day -1. Bleeding was performed at days 8, 21, and 30. Serum lactate dehydrogenase (LDH) was determined enzymatically. The titer of Ab anti-OVA was determinated by ELISA, whereas the proportion of Ab directed to native OVA epitopes was calculated by ELISA competition assays. Results: MAb to IL-17A decreased the plasmatic LDH levels induced by LDV (3668±547 and 2900±175 U/L, for control and MAb treated mice, respectively, P