Chlorpyrifos promotes cell invasiveness and activates P-38/GSK3Β pathway mediated by ROS increment in breast cancer cell lines

MARIANELA LASAGNA; A RANDI; MIRET N; CLAUDIA COCCA; VENTURA CLARA; NUÑEZ, MARIEL

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

Chlorpyrifos (CPF) is one of the most common pesticide used for control pest in agriculture in our country. Previously, we reported that 0.05 uM CPF induced cell proliferation in an estrogen receptor dependent way in MCF-7 cells and 50 uM CPF inhibited cell proliferation via p-ERK induced by an increment of reactive oxygen species (ROS) in MCF-7 and MDA-MB231 cells. Objective: Our aim was to analyze if CPF may promote cell invasion and the associated signalingin breast cancer cell lines. Methods: MCF-7 and MDA-MB231 cells were exposed or not to CPF (0.05 or 50 uM). Cell invasiveness was examined by the Matrigel® invasion assay and c-SRC, p38 and GSK3β phosphorylations by Western Blot. Results: 50 uM CPF induced the invasion in MDA-MB231 after 24 h, (60% over control, p< 0.01) and MCF-7 cells after 48 h (400% over de control, p< 0.001).CPF at 0.05 and 50 uM increased c-SRC phosphorylation (p-cSRC) after 60 min in MCF-7 cells (19% and 14% over control respectively, p< 0.05) and this effect was reverted by catalase (30 IU/mL). In MCF-7 cells, 50 uM CPF enhanced phospho-p38 (p-p38) after 30 minutes of exposure (27% over control, p