IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LITHIUM AND BONE MARROW MONONUCLEAR CELLS: COMBINED THERAPY IN THE TREATMENT OF SCIATIC NERVE REVERSIBLE DEMYELINATION
Autor/es:
CLARA P. SETTON; PAULA A, SOTO; ALICIA CUETO; GONZALO PIÑERO; PAULA V. MONJE; VANINA USACH
Lugar:
CABA
Reunión:
Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017
Resumen:
Lithium salts have been the conventional pharmacological treatment for bipolar and major depressive disorders for over six decades. Additional benefits of lithium include neuroprotective, anti-inflammatory, and anti-apoptotic effects, which have been exploited to treat central nervous system trauma and chronic neurodegenerative diseases. In the peripheral nervous system, lithium administration has been shown to foster functional recovery and remyelination after injury. Our group has studied the bioactivity, specificity, and reversibility of lithium?s action on cultured Schwann cell (SC) growth, survival, proliferation and differentiation, in addition to basal lamina and myelin formation in SC-neuron cultures.On the other hand, our group has demonstrated, in a model of sciatic nerve crush that systemically transplanted bone marrow mononuclear cells (BMMC) spontaneously migrate to and remain in the injured nerve for as long as 60 days post injury (dpi). Once in the ipsilateral nerve, some of the cells colocalized with SC markers. BMMC were also shown to exert a beneficial effect on axon regeneration and remyelination and prevent hyperalgesia.In the present work, we combine these two potential treatments with the aim of improving nerve recovery. After nerve compression, animals received intravenous transplantation of BMMC and orally administered lithium chloride. Axonal regeneration, remyelination and functional recovery were analyzed 14 dpi. While combined therapy did not exhibit differences regarding BMMC treatment in terms of myelin recovery, analyzed through immunohistochemistry or western blot (p