IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A neurosphere approach to revisit the cuprizone model
Autor/es:
SILVESTROFF, L; MOLINARI, Y; FRANCO, P
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias. LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2017
Institución organizadora:
SAIC
Resumen:
Cuprizone (CPZ) intoxication in rodents has been used for many years as an experimental model of demyelinating diseases such as Multiple Sclerosis. CPZ in vivo effects in the CNS include astrogliosis, microglial activation and the progressive loss of oligodendrocytes (OL) throughout the brain resulting in demyelination and neurotoxicity. The mechanisms by which CPZ promotes oligodendroglial loss has not been fully understood yet. Initial stages of CPZ intoxication are accompanied by the activation of cell proliferation in the Subventricular zone (SVZ). Coincidently, OL precursor cell (OPC) numbers are also strongly upregulated in several brain areas during the first stages of demyelination. These first attempts to balance out OL deficit seem to be unsuccessfull, since OPC cannot progress towards mature myelinating OL and progressively die by apoptosis. Here, by using an in vitro model of oligodendrogenesis from neurospheres (NS), we studied CPZ effects on NSC/NPC proliferation and survival, cell migration and cell differentiation.