IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Sphingosine Kinase 2 preserve epithelial barrier during cell diferentiation
Autor/es:
TALLARO S; SANTACREU BJ; STERIN SPEZIALE NB; ROMERO DJ; FAVALE NO
Lugar:
CABA
Reunión:
Congreso; II Reunión Conjunta de Sociedades de BioCiencias.; 2017
Institución organizadora:
SAIB-SAIC-SAI-SAA-SAB-SAB-SAFE-SAFIS-SAH-SAP
Resumen:
Sphingosine 1-Phophate (S1P) is a sphingolipid mediator of cellu- lar fate. We have demonstrated that hypertonicity induces epithelial tissue organization by the establishment of mature adherent junc- tions. Organization and preservation of epithelial tissue requires an ef cient cell extrusion followed by a closing of cellular gap to pre- serve tissue permeability. The aim of the work was to investigate the participation of sphingosine kinase (SK)/S1P pathway in the cellular extrusion in differentiated MDCK cells. For this, con uent MDCK cells were subjected to hypertonic medium with the concomitant knock down of SK1, SK2 or not (control) by siRNA, or pharmacolog- ical inhibition of SK. After 48 h, cell phenotype was visualized by u- orescence microscopy, evaluating actin cytoskeleton and Adherens Junction (AJ) formation. By performing a confocal z-plane reconstruction we visualized extruding cells and found that the inhibition of SK evoked an alteration in the cell extrusion. SK2 knock-down produced de cient cell extrusion in live cell microscopy while SK1 knock-down do not shown any effect in the procces. We also found that SK inhibition impars the E-cadherin fragmentation necessary for cell detachment before the extrusion. In conclusion, we observe that SK/S1P pathway is involve in the cell extrusion procces and depends on SK2 activity.