IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Polyamines reduced growth by modulating reactive oxygen species and nitric oxide formation in wheat
Autor/es:
GROPPA M.D.; VÁZQUEZ A.; RECALDE L.; BENAVIDES M.P.
Lugar:
Córdoba
Reunión:
Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
Resumen:
Polyamines (PAs), compounds involved in plant development, are able to generate reactive oxygen species (ROS) and nitric oxide (NO) during their normal catabolism. PAs increased levels in plants have usually been related to abiotic stress tolerance. However, PAs are also well known to induce PCD or alter growth or development upon depletion/overproduction with respect to their physiological levels. The aim of this work was to evaluate If ROS and NO were involved in wheat growth inhibition after the exogenous addition of 1 mM putrescine (Put), spermidine (Spd) or spermine (Spm) or 100 µM of sodium nitroprusside (SNP) as NO donor. All PAs and NO significantly reduced seedling growth and increased NO formation. SNP and Spm produced the most drastic reduction in growth, and almost completely inhibited O2.-formation, increasing H2O2 levels. Catalase-dependent H2O2 removal reversed SNP-induced growth repression and restored O2.-generation, whereas the NO scavenger, cPTIO, recovered 50% of root length when was added with Spd, Spm or SNP. Aminoguanidine (DAO inhibitor) and 1,8-diaminooctane (PAO inhibitor) restored growth in the presence of SNP and Put. Wheat growth reduction occurred after PAs or NO exposure was mediated by an altered balance among the O2.-, H2O2 and NO, produced by a modified NADPH oxidase activity and/or to an increased PAs catabolism mediated by DAO or PAO enzymes