IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Overexpression of the Parkinson-associated Spf1 protein.
Autor/es:
DELLES DENISE; MAZZITELLI LR; BLANCO M LUCIA; ADAMO HP; CORRADI GC
Lugar:
CABA
Reunión:
Congreso; III Congreso Internacional de Medicina Traslacional; 2016
Resumen:
The Spf1 protein is one of the two P5-ATPases present in the yeast Saccharomyces cerevisiae. Loss of function mutations in the human homologs of the P5-ATPases has been associated with neurodegeneration and early-onset Parkinson?s disease. The Spf1 is presumed to be involved in the ATP fueled transport of a yet unknown substance toward the lumen of ER and endocytic vesicles. In agreement with this idea we have shown that the purified rSpf1 is capable of forming the characteristic phosphoenzyme and this reaction is inhibited by micro molar concentrations of Ca2+. Here we have investigated how the growth of yeast is affected by the knock out (KO) and overexpression of Spf1 (OE) when the culture medium contains various concentrations of drugs altering the calcium homeostasis. Because in yeasts Ca2+ homeostasis in the ER is dependent on the vesicular traffic, we have tagged the mitochondria and the endocytic compartment by expressing mtGFP and Cerulean Rab homolog YPT7, respectively, which will allow us to investigate the effect of Spf1 on the function of these compartments.