Design and characterization of liposomes containing stimuli-responsive molecules.

CARBALLO ROMINA; FLOR SABRINA ; SOTO ESPINOZA SILVIA; IRENE REZZANO

Tucuman

Congreso; II LAFeBS-IX Ibero American Congress of Biophysics- XLV SAB.; 2016

LAFeBS/SAB

Liposomes prepared from egg yolk lecithin (EYL) and dipalmitoylphosphatidylcholine (DPPC), were doped with selected metalloporphyrins Our goal is to develop liposomes as electrochemical sensor by binding of different molecules and explore their potential applications such as sensor devices and photodynamic therapy. We propose study two different modified molecules (ferrocene and protoporphyrins esterified with octadecylamine, namely FC-ODA and Ppix-ODA) and compare the performance with unmodified molecules. Porphyrin is useful for photodynamic therapy treatment [1]. Moreover, ferrocene-modified liposomes are ideal biomimetic membrane models that present straightforward electrochemical behavior and stability [2]. Liposomes were synthesized as describes [3]. A solution of Ppix and Ppix-ODA 3.3 µM were added to the liposome containing samples (in ratio 1:5) and were mixed on a shacker for 6 h to reach full binding equilibrium. However, Fc and Fc-ODA were added during liposome synthesis. In the present work we analysed the behaviour of liposomes binding of different molecules. The response was measured by electrochemical or fluorescence signal, electrophoretic mobility and UV spectroscopy. Different behaviours were found for different systems evaluated.