Overexpression of the Parkinson-associated Spf1p P5 ATPase

MARIA LUCIA BLANCO; ADAMO HUGO PEDRO; DELLES DENISE; CORRADI GERARDO RAUL; MAZZITELLI LUCIANA ROMINA

Buenos Aires

Congreso; III Congreso Internacional de Medicina Traslacional; 2016

Maestría Internacional en Ciencias Biomédicas (IMBS).Facultad de Farmacia y Bioquimica, UBA, Buenos Aires, Argentina. Universidad de Friburgo, Alemania.

Overexpressionof the Parkinson-associated Spf1 protein.Denise Delles, M. LuciaBlanco,  Gerardo R. Corradi,  Luciana R. Mazzitelli, and Hugo P. AdamoInstituto de Química yFisicoquímica Biológicas (IQUIFIB) Departmento de Química Biológica, Facultadde Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 BuenosAires, Argentina. The Spf1protein is one of the two P5-ATPases present in the yeast Saccharomyces cerevisiae. Loss of function mutations in the humanhomologs of the P5-ATPases has been associated with neurodegeneration andearly-onset Parkinson?s disease. The Spf1 is presumed to be involved in the ATPfueled transport of a yet unknown substance toward the lumen of ER andendocytic vesicles. In agreement with this idea we have shown that the purifiedrSpf1 is capable of forming the characteristic phosphoenzyme and this reactionis inhibited by micro molar concentrations of Ca2+. Here we haveinvestigated how the growth of yeast is affected by the knock out (KO) andoverexpression of Spf1 (OE) when the culture medium contains variousconcentrations of drugs altering the calcium homeostasis. Because in yeasts Ca2+homeostasis in the ER is dependent on the vesicular traffic,  we have tagged the mitochondria and theendocytic compartment by expressing mtGFP and Cerulean Rab homolog YPT7,respectively, which will allow us to investigate the effect of Spf1 on thefunction of these compartments. Acknowledgments: This work is being supported by ANPCyT, UBAand CONICET. D.Delles is an exchange student .