IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Notch activation during CNS demyelination-remyelination: time- and cell type-dependent ligand expression.
Autor/es:
DANA ESQUENAZI; DÉBORA RODRIGUEZ; PATRICIA MATHIEU ; LAURA GÓMEZ PINTO; ANA M. ADAMO
Reunión:
Congreso; 2° Congreso de la Federación Latino americana y del Caribe de las Sociedades de Neurociencias (FALAN); 2016
Resumen:
CNS demyelination is a pathological process characterized by myelin loss around axons, while subsequent remyelination solves functional deficits. In this context, the current work studied Notch1 ligand differential roles in a cuprizone (CPZ)-induced demyelination model. Adult Wistar rats were fed a CPZ or control diet for 2 weeks and sacrificed 7d before (-7d), upon (0d), and 7, 14 and 21d (+7d, +14d and +21d) after CPZ withdrawal. Immunohistochemical studies in the CC rendered a significantly higher percentage of Notch ligand Jagged1+ cells in CPZ-treated animals than in controls at -7d, 0d and +7d, while no significant differences between groups were observed at +14d and +21d. Cells expressing Jagged1 were all found to be GFAP+, while none of them were characterized as NG2+ or Olig2+. Studies in the CC also revealed a greater proportion of F3+ cells in CPZ animals than in controls at -7d, 0d and +7d and no significant differences between groups as from +14d. Cell type characterization showed all F3+ cells to be either NG2+ or Olig2+, as both F3+/NG2+ and F3+/Olig2+ proportions were found to be significantly larger in CPZ animals than in controls at 0d and +7d. Also worth mentioning, none of the F3+ cells were found to be GFAP+. Western blot analyses conducted in the CC and SVZ provided support for immunohistochemical findings. Taken together, these results prove changes in Notch ligand expression upon demyelination to be time- and cell type-dependent.