IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Sphingosine 1 Phosphate Receptor 2 Regulates Epithelial Cells Monolayer Integrity
Autor/es:
FREIRE PT; ROMERO DJ; FAVALE NO; SANTACREU BJ
Lugar:
Córdoba
Reunión:
Congreso; LII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2016
Institución organizadora:
SAIB
Resumen:
Sphingosine 1-Phophate (S1P) is a sphingolipid mediator involved in cellular fate that acts both extracellularly as ligand for cell surface receptors (S1PR), and intracellularly as second messenger. We have demonstrated that hypertonicity induces epithelial cell differentiation which depends on changes in sphingolipid metabolism. Epithelial cell differentiation needs a specific intracellular organization as well as the correct monolayer formation. This process requires an adequate removing of apoptotic cells followed by a rapid closes of empty spaces, thus preserving tissue permeability and integrity. This physiological process is known as cell extrusion and is related to S1P cellular effects. We found that in differentiated epithelial cultures cells, extrusion was dependent on S1P synthesis, the activation of S1PR2/Rho pathway and plasma membrane (PM) S1PR2 localization. To evaluate the importance of S1PR2 localization we obtained cells that overexpress S1PR2 with PM localization. In this condition, confluent cells (and non- differentiated) were extruded from the monolayer. Interestingly, the extrusion was independent of cell apoptosis and the cells were extruded as a dome, with lost of cell-matrix adhesion and the preservation cell-cell adhesion. These results show that SK/S1P pathway has multiple functions that not only dependent on the levels of S1P.