Identification and characterization of the signaling pathways triggered by the mas receptor

QUIROGA DT; DOMINICI FP; BURGHI V; FERNÁNDEZ NC; ECHEVERRÍA E

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC),; 2016

The Mas receptor (MasR) is a class A Orphan G-protein coupled receptor (GPCR). Although angiotensin-(1-7) [Ang-(1-7)] has been reported as its putative ligand, the intracellular signaling pathways activated by the MasR remain partially characterized preventing definitive knowledge of ligand/receptor pharmacology.In this study we examined MasR-dependent activation of canonical (G protein-mediated) and non-canonical (MAP kinase mediated) GPCRs? signaling pathways. With that aim, we used endogenously expressing human lung carcinoma A549 cells to evaluate Gq-coupled activity, as it was previously described by other authors, and we monitored changes in intracellular Ca2+ levels after Ang(1?7)-stimulation. Incubation with 100nM Ang(1-7) failed to increase Ca2+ levels either in endogenously expressing or MasR transfected A549 cells. On the other hand, transfection of HEK293T cells with MasR resulted in a significant decrease in basal cAMP levels (p