IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Absence of endogenous Galectin-1 does not affect the normal axonal development
Autor/es:
PASQUINI JM; BARRANTES FJ; QUINTÁ HR
Reunión:
Congreso; Sociedad Argentina de Investigación Clinica; 2016
Resumen:
During the embryonic stages, the axonal development is crucial to establish the neuronal connections, which among other things will allow normal functionality of locomotor activities. We have already shown that Galectin-1 (Gal-1) treatment in dimeric form promotes reactivation of actin cytoskeleton dynamics and therefore axonal re-growth after a spinal cord injury (Quinta et al., 2016). Further, we observed that repair phenomenon does not take place with monomeric Gal-1 treatment (Quinta et al., 2014a, b) which structure correspond to endogenous concentrations of this lectin, 7 mM. Taking into consideration that endogenous Gal-1 could not modify the actin cytoskeleton dynamics our objective is to determine whether the complete lack of endogenous Gal-1 affect the axonal development. Using embryonic lgals-1-/- mice we determined the 3D axonal development plus axonal number and axonal length in comparison with observed in wild type (WT) and lgals-3-/- embryos. We used a novel optical technique which allows acquire 3D images using whole embryos combining CLEARING technique (Quinta et al., 2015) plus 1-Photon microscopy. Moreover, we identified single axons and its sprouting deep in the tissue at high magnification without tissue sectioning by performing a high-sensitive 3D-epifluoresence microscopy. Our results show that absence of endogenous Gal-1 did not affect the normal axonal development, showing similar axonal length that was observed in WT and lgals-3-/- embryos. Besides there were not no significant difference in the number of axons from dorsal root ganglia and in the shape of axonal growth cone regarding that were observed in the wild type embryos. Finally, the presence of PlexinA4, main axonal guidance protein, did not changed by the absence of endogenous Gal-1.In conclusion, these results confirm that the absence of endogenous Gal-1 does not modify the axonal cytoskeleton dynamics and the normal development, supporting our previous results.