IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cuprizone effects on proliferation and differentiation of neural stem cells
Autor/es:
MOLINARI, Y; FRANCO, P; SILVESTROFF L
Lugar:
Mar del Plata
Reunión:
Congreso; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias.; 2015
Institución organizadora:
SAIC
Resumen:
Cuprizone effects on proliferation and differentiation of neural stem cells Cuprizone (CPZ) is a copper-chelating agent which induces demyelination in mice. Although its neurotoxic mechanism is still unknown, it was shown in vivo that CPZ produces astrogliosis, microglial activation and loss of oligodendrocytes that progressively lead to demyelination and neuronal degeneration.Neural stem and progenitor cells (NSC/NPC) are able to generate all neural cell types and can be cultured as Neurospheres (NS). Depending on culture conditions, NS can be maintained in a proliferative and undifferentiated sate or alternatively they can be forced to differentiate into neurons, astrocytes and oligodendrocytes. Here, by using NS cultures, we evaluate CPZ effects on NSC/NPC survival and proliferation, cell migration and cell differentiation.Although NSC/NPC survival was not affected at lower CPZ doses, we detected a reduction in NSC/NPC viability at higher CPZ concentrations. Cell proliferation was not affected in the presence of CPZ at any tested concentrations. Contrarilly, differences in cell migration patterns were found in CPZ treated cultures in comparison to control cells.CPZ treatment of dissociated NS during differentiation process did not change neurons or astrocytes proportions. However, oligodendroglial differentiation was completely abolished when cells were exposed to low CPZ doses. The detection of oligodendroglial precursor cells in these conditions, suggests that CPZ has an inhibitory effect on oligodendroglial maturation.