IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of ion concentration on the interaction dengue virus's NS3-ssARN
Autor/es:
LA CABABIE; JJ INCICCO; LG GEBHARD; AV GAMARNIK; RM. GONZÁLEZ LEBRERO; SB. KAUFMAN
Lugar:
Salto
Reunión:
Congreso; Latin American Crosstalk In Biophysics And Physiology; 2015
Institución organizadora:
Seccional Biofísica de la Sociedad Uruguaya de Biociencias (SBF.uy), la Sociedad Argentina de Biofísica y LAFeBS
Resumen:
The dengue virus´s NS3 protein is a helicase that catalyzes the hydrolysis of ATP and couples the free energy of this reaction to drive translocation on single strands and unwinding double strands of RNA. Most protein-nucleic acid interactions are extremely sensitive to the concentration of salts in solution. This is because linear nucleic acids are highly charged polyanions and as a result sequester cations in their vicinity in order to reduce the net charge on the nucleic acid. It has been postulated that association constant (Kobs) for protein-nucleic acid interaction decreases with increase salt concentration. Binding protein-nucleic acid is potentiated by the increase entropy originate for the ions release to free solution1.In this work we studied the effect of cations on the interaction between DENV NS3 and ssRNA. We performed titration curves NS3 / RNA in the presence of different concentrations of K+, Na+ and Mg2+ using a fluorescent 10 base-RNA oligonucleotide. In all cases, we observed that Kobs decreases with salt concentration. We could analyse our results with Manning theory[1] and obtein the number of ions release from the ssRNA in the reaction. 1, Manning, G. S.  Quarterly reviews of biophysics, 11(02), 179-246, 1978With grants from Universidad de Buenos Aires (UBACyT), CONICET, and ANPCyT