IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
INTERLEUKIN 17 (IL-17) IS INVOLVED IN SOME EFFECTS OF MURINE HEPATITIS VIRUS (MHV-A59)
Autor/es:
APARICIO, JL; DUHALDE VEGA M,; RETEGUI, LA
Lugar:
Buenos Aires
Reunión:
Congreso; IV LASID meeting; 2015
Institución organizadora:
LASID-SAI-FAIC
Resumen:
Background: MHV-A59 triggers various mouse pathologies such as hepatitis, thymus involutionand hypergammaglobulinaemia. We reported that viral infection induced autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH) and release of uric acid and high-mobility group box protein 1. Since IL-17 is a pro-inflammatory cytokine involved in autoimmune diseases, cancers and viral infections, we explored the effects of neutralizing IL-17 with a monoclonal antibody (MAb MM17F3) on MHV activity. Methods: C57BL/6 mice were infected with MHV-A59 (104 TCID50) and 4, 7 and 11 post-infection were inoculated with 150 µg of anti IL-17 MAb (MM17F3). After infection, serum transaminases and uric acid concentration were determined enzymatically, immunoglobulins, IL-17 and IL-6 were measured by sandwich ELISA, and autoAb to FAH were detected by Western-blot. Liver histopathology was also explored. Results: Treatment of animals with MAb MM17F3 increased the plasmatic IL-17 half live. However, neutralization of IL-17 decreased the level of transaminases (P