Sphingomyelin Synthesis Is Involves In Ephithelial-Mesenchymal Transition Process

FAVALE NO; SANTACREU B; PESCIO LG; MARQUEZ MG; STERIN-SPEZIALE NB

Aberdeen

Congreso; 55th International Conference on the Bioscience of Lipids (ICBL); 2014

ICBL

We have previously demonstrated that sphingomyelin biosynthesis is essential for hypertonicity-induced MDCK differentiation. Under inhibition of SM synthesis, MDCK cells instead to differentiate switch to mesenchymal phenotype thus performing an ephitelial to mesenchymal transition. We aim to study the sphingolipid metabolic pathway as well as the sphingomyelin synthase isoform involved in such a process. Confluent MDCK cells were submitted to hypertonicity and concomitantly SMS was inhibited by pharmacological and knockdown strategy (D609 or siRNA-SMS1). Both strategies showed alteration of polarized phenotype with acquisition of messenchymal phenotype. To evaluate the ephithelial-mesenchymal transition (EMT), different marker was performed. The results showed an increase in mesenchimal marker suck as Vimentin and the transcription factor Snail-1. On the other hand was accompanied by the loss of the epithelial marker, particularly the kidney specific cadherin (cad16). These results demonstrate implication of SMS1 in the EMT by the Snail-1. Is important to note that SMS1 activity was involved not only in the phenotype acquisition, moreover, was involved in gene expression.