Effects of phosphoatidylcholine-bile salt nanoparticles over MCF-7 breast cancer cell.

GÁNDOLA YB; FERIOLI S; PÉREZ SE; CARLUCCI AM; GONZÁLEZ L,

Rosario, Santa Fe

Congreso; L Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB).; 2014

L Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB).

Phosphatidylcholine-bile salt mixed micelles are nanoparticulated systems extensively studied and proposed for the delivery of therapeutics. A recent trend in nanotechnology has been to investigate the interactions of nanoparticles with biological systems, since their effects might depend on their properties (i.e., chemical composition). The aim of the present work was to analyze the effects over cell viability and signaling mediators, of soybean phosphatidylcholine (SPC) and sodium cholate (SC) mixed micelles prepared at different SPC:SC molar ratios (1:1, 1:2, 1:4, 1:8) over the MCF-7 cell line. Cell viability was assessed by the MTT cell viability assay and Erk1/2 and Akt activation was determined by western blotting. Results showed that mixed micelles containing the highest proportion of SC are citotoxic after 24 hs incubation. Increased phosphorylation of Erk1/2 and Akt after 24hs incubation correlated with the cytotoxic effects. To ascertain if the described effects accounted on SC, cell viability and activation of Erk1/2 and Akt was assessed after incubation with different SC concentrations. In accordance with results found for the MM containing high SC proportion, SC at high concentration was cytotoxic and induced activation of Erk1/2 and Akt. Sustained activation of the kinases might be involved in the cytotoxic effect of high SC concentrations over breast cancer cells.