IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bradykinin (Bk) Favours The Formation Of Migratory Colonies Of Nephrogenic Ureteric Bud (Ub) Cells
Autor/es:
GUAYTIMA E; BRANDAN YR; MEGÍAS FE; FAVALE N; STERIN-SPEZIALE NB; MARQUEZ MG
Lugar:
ROSARIO, SANTA FE
Reunión:
Congreso; Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
In mammals, nephrogenesis is completed posnatally. We have shown that primary cultures of renal papillary UB cells of neonatal rats-which give rise to the collecting duct-, display a phenotype of migratory sheet of epithelial cells. Now we explore the implication of BK in these phenomena. We examined by immunofluorescence the cellular distribution of F-actin and vinculin (Vin). After 58h, cells formed low compacted colonies making contacts with neighboring cells through filopodium and interacting with the substratum through Vin positive focal adhesions. F- actin cell cortex and stress fibers were also observed. After BK treatment the colonies packed, typical of epithelial cells, and Vin-stained cell-cell adhesions appeared. A continuous F-actin belt along the outer contour of the colonies and ruffling-lamelipodium were observed, reflecting the formation of migratory colonies. Pre-treatment with a selective BK-B2 receptor (B2R) antagonist avoided the BK-induced cell-cell adhesion and F-actin colony delineation. Pre-treatment with a PI3K inhibitor impaired the BK?induced cell compactation and ruffle formation. Since B2R blockade results in abnormal tubular differentiation, we propose that in kidney development, BK favors cell compactation to form migratory colonies, and this behavior could explain the collective advance of the UB from papilla to renal cortex during nephrogenesis.