Demyelination-remyelination in the CNS: ligand-dependent participation of the Notch signaling pathway

FLORENCIA ALMEIRA GUBIANI; MILAGROS PEREIRA LUPPI; PATRICIA MATHIEU; ANA M. ADAMO

Huerta Grande. Córdoba

Congreso; XXIX Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2014

Sociedad Argentina de Investigación en Neurociencia

In the CNS, myelination is a physiological process driven by oligodendroglial cells, while demyelination is a pathological process characterized by myelin loss around axons. Demyelination is followed by remyelination, thus solving functional deficits. This work focused on the study of Notch1 ligands, and their role in demyelination-remyelination process in a CPZ-induced demyelination model. Twenty-one-day-old Wistar rats were fed with a diet containing CPZ during 2 weeks. Demyelinated and control animals were sacrificed 7d before CPZ withdrawal (-7d), the day of CPZ withdrawal (0d), 7, 14 and 21d after CPZ withdrawal. Experiments were conducted for each survival time. We determined the levels of F3 by WB and characterized F3 expressing cells by IHC in the subventricular zone (SVZ) and corpus callosum (CC) of control and CPZ animals. We also characterized cell populations in primary neurosphere culture from SVZ of control and CPZ animals. Results showed an increase in Olig2+ cells expressing F3 at -7d, 0d, +7d and +21d in the CC and SVZ of CPZ animals as compared to controls. Regarding the neurosphere culture, preliminary results showed an increase in Jagged-1+ cells in CPZ animals at -7d without changes in F3+ cells. Regardless at 0d the percentage of F3+ cells increased along with a decrease in Jagged-1 + cells. These results reinforce the notion of F3/contactin involvement in OPC differentiation during the remyelination process.