Reduced glomerular number as a potential mechanism underlying hypertension programming in male offspring from mothers with adenine-induced chronic kidney disease

MAGNANELLI, A; SUCHOWOLSKI, G; GAYONE, S; ALONSO, R; MAJOWICZ, MP; ORONEL, L; JENCQUEL, C; ALBERTONI BORGHESE, MF

Mar del Plata

Congreso; Reunion anual de sociedades de biociencias; 2023

Sociedad Argentina de Investigacion clinica

At least 3-4% of women in reproductive age present Chronic Kidney Disease (CKD)and pregnant women with CKD have an increased risk of perinatal complications.However, limited attention has been given to the potential effects of maternal CKDon offspring. The maternal Adenine-induced CKD (m-Ad-CKD) rat model is veryrecent and unexplored. It was reported that adult male rat offspring of m-Ad-CKDdevelop hypertension in adulthood.Our aim was to evaluate glomerular count in 21 days old rat offspring from m-Ad-CKD, bearing in mind that low glomeruli number is one of the mechanismsunderlying hypertension programming.Adult female SD rats were treated for 3 weeks with Ad 0.5% in standard rat chowpowder (CKD mothers=CKDM) or regular rat chow powder (Control mothers=CM).Then, they were mated with male SD rats.Offspring number from CKDM was lower than that from CM (8 vs 14). Litter size ofthe CM was fixed at 10 pups. The offspring was divided into 4 groups: male from CM(M CM ;n=5); male from CKDM (M CKDM ;n=3), female from CM (F CM ;n=5), and female fromCKDM (F CKDM ;n=5). After weaning (day 21), pups were weighed and sacrificed. Bloodand urine samples were obtained to determine plasma and urinary creatinine andproteinuria. The right kidney of each pup was used to count glomerular number bythe acid maceration method. Results were expressed as media ± SD and 2-wayANOVA was used for statistics.We found a reduced glomerular number in M CKDM (73881±34347) vs M CM(105895±28111) and vs F CKDM (96454±28520) with p