Bone marrow mononuclear cells may provide an endogenous repair mechanism in the demyelinated sciatic nerve.

USACH V; LOPEZ MARGARITA; LAVALLE LUCIA; MARTINEZ VIVOT ROCíO; BRUSCO ALICIA; SETTON CP

Huerta Grande, Córdoba

Congreso; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2011

Sociedad Argentina de Investigaciòn en Neurociencia

In previous work, we have demonstrated the reorganization of major myelin proteins P0 and MBP and axonal protein PGP 9.5 in the demyelinated area, as well as the migration of CD34+ bone marrow mononuclear cells (BMMC) during Wallerian degeneration in rats’ sciatic nerves. Once in the demyelinated area, some BMMC conserve their phenotype while others change it to S100â+, MBP+ and PGP9.5+. In the present work we evaluated prostaglandin (PG) participation in cell recruitment through Western blot and BMMC possible effect on remyelination through electron microscopy and immunohistochemistry. As regards PG analysis, the expression of Cox1 showed an increase 6 h after lesion, while Cox2 expression was induced 6 h after nerve injury and continued until 24 h, which suggests PG as one of the biological signals involved in BMMC recruitment. On the other hand, a reduction was observed in MBP and P0 clusters, as well as the presence of more myelinated axons in the crush area in BMMC-transplanted rats, although their myelin structure was still different from control axons. These data suggest BMMC beneficial effect on myelination either by preventing demyelination or stimulating remyelination. Yet, further experiments will be necessary to elucidate the mechanisms involved in BMMC migration.