IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Neurogenesis in the zebrafish retina: glutamatergic control of cell proliferation
Autor/es:
CARLOS DAVID BRUQUE; DIEGO VILLAGRA; MARIA PAULA FAILLACE
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVI Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2011
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias (SAN)
Resumen:
Neurogenesis in the zebrafish retina: glutamatergic control of cell proliferation Carlos Bruque1, Diego Villagra1, M. Paula Faillace1,2 1Departamento de Fisiología, FMED, UBA  2IQUIFIB-CONICET The retina of zebrafish grows throughout animal’s life from an intrinsic germinal region at the periphery of the retina called ciliary marginal zone (CMZ). This animal model allows studying cell proliferation and differentiation processes to generate new cell types of the adult retina. On the other hand, glutamatergic transmission from photoreceptors to bipolar cells (BC) and from BC to ganglion cells (GC) mediates light processing and information transmission to the visual brain centers. We blocked the depolarization of ON BC, which are activated in response to light, through an agonist (L-AP4) of mGluR6 receptors, whose activation by glutamate hyperpolarizes ON BC. We also used an antagonist of AMPA receptors DNQX, to block excitatory synapses between photoreceptors and OFF BC and between BC and all GC (although part of the response is NMDA receptor-mediated). DNQX treatment significantly increased proliferating cell number (measured by BrdU incorporation in the CMZ). Moreover, a still non significant increase of L-AP4 in cell proliferative activity has been observed. Therefore, these and previous results might indicate a relevant role of glutamate (and hence light and dark signals) in regulating mitotic activity and cell addition for retinal growth in the adult zebrafish.