IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Neurogenesis in the zebrafish retina: glutamatergic control of cell proliferation
Autor/es:
CARLOS DAVID BRUQUE; DIEGO VILLAGRA; MARIA PAULA FAILLACE
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVI Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2011
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias (SAN)
Resumen:
Neurogenesis in the zebrafish retina: glutamatergic control of cell
proliferation Carlos Bruque1, Diego Villagra1,
M. Paula Faillace1,2
1Departamento
de Fisiología, FMED, UBA
2IQUIFIB-CONICET
The retina of zebrafish grows throughout
animals life from an intrinsic germinal region at the periphery of the retina
called ciliary marginal zone (CMZ). This animal model allows studying cell
proliferation and differentiation processes to generate new cell types of the
adult retina. On the other hand, glutamatergic transmission from photoreceptors
to bipolar cells (BC) and from BC to ganglion cells (GC) mediates light processing
and information transmission to the visual brain centers. We blocked the
depolarization of ON BC, which are activated in response to light, through an
agonist (L-AP4) of mGluR6 receptors, whose activation by glutamate
hyperpolarizes ON BC. We also used an antagonist of AMPA receptors DNQX, to block
excitatory synapses between photoreceptors and OFF BC and between BC and all GC
(although part of the response is NMDA receptor-mediated). DNQX treatment significantly
increased proliferating cell number (measured by BrdU incorporation in the CMZ).
Moreover, a still non significant increase of L-AP4 in cell proliferative
activity has been observed. Therefore, these and previous results might
indicate a relevant role of glutamate (and hence light and dark signals) in
regulating mitotic activity and cell addition for retinal growth in the adult
zebrafish.