IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Thermal regulation of membrane lipid fluidity by a two component system in Bacillus subtilis
Autor/es:
BREDESTON LUIS M; MARCIANO DANIELA; ALBANESI DANIELA; DE MENDOZA DIEGO; DELFINO JOSE M
Revista:
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
Editorial:
JOHN WILEY & SONS INC
Referencias:
Lugar: Hoboken, NJ; Año: 2011
ISSN:
1470-8175
Resumen:
This article describes a simple and robust laboratory exercise on the regulation of membrane unsaturated fatty acid composition in bacteria by a decrease in growth temperature. We take advantage of the well characterized Des pathway of Bacillus subtilis, composed of a D5-desaturase (encoded by the des gene) and the canonical two component system DesK-DesR, to study the transcriptional regulation of des during cold shock. Students analyze the expression of a reporter transcriptional fusion between the des promoter and the bacterial lacZ gene in a wild-type B. subtilis strain and in des or desK-desR mutants grown under different culture conditions. â-galactosidase activity measurements allow them to investigate how the Des pathway works and to assess the role of each component of this regulatory system.