Novel cellular mechanism that mediates the collecting duct formation during postnatal renal development

FAVALE, NICOLÁS OCTAVIO; GUAYTIMA, EDITH; STERIN - SPEZIALE, NORMA B.; BRANDÁN, YAMILA ROMINA; MÁRQUEZ, MARÍA GABRIELA

JOURNAL OF CELLULAR PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2019 vol. 234 p. 13387 - 13402

0021-9541

We have previously demonstrated that kidney embryonic structures are present in rats,and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, therat renal papilla contains newly formed collecting duct (CD) cells and others in a moremature stage. Performing primary cultures, combined with immunocytochemical andtime‐lapse analysis, we investigate the cellular mechanisms that mediate the postnatalCD formation. CD cells acquired a greater degree of differentiation, as we observed thatthey gradually lose the ability to bind BSL‐I lectin, and acquire the capacity to bindDolichos biflorus. Because CD cells retain the same behavior in culture than in vivo, and byusing DBA and BSL‐I as markers of cellular lineage besides specific markers of epithelial/mesenchymal phenotype, the experimental results strongly suggest the existence ofmesenchymal cell insertion into the epithelial CD sheet. We propose such a mechanismas an alternative strategy for CD growing and development