Beneficial activity of Enteroccocus faecaeis CECT7121 in the anti-limphoma protective response

M.S. CASTRO; M.A. MOLINA; P. DI SCIULLO; M.B. AZPIROZ; F. LEOCATA NIETO; N.B. STERIN-SPEZIALE; C. MONGINI; M.A. MANGHI

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2010 vol. 5072 p. 1234 - 1243

1364-5072

Abstract Aims: To study the anti-tumour effects of Enterococcus faecalis CECT7121 on LBC cells, an aggressive murine T-cell lymphoma that kills the host in 18 days when is intraperitoneally (i.p.) administrated.To study the anti-tumour effects of Enterococcus faecalis CECT7121 on LBC cells, an aggressive murine T-cell lymphoma that kills the host in 18 days when is intraperitoneally (i.p.) administrated. Methods and Results: In vitro studies have shown that LBC cell proliferation was inhibited by Ent. faecalis CECT7121 stimulus in a dose-dependent manner, inducing apoptosis. The production of ceramide was involved in the latter effect. To undertake in vivo studies, syngeneic BALB ⁄ c mice pre-treated i.p. with Ent. faecalis CECT7121 (2Æ5 · 108 CFU) were challenged i.p. with LBC cells (1Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.In vitro studies have shown that LBC cell proliferation was inhibited by Ent. faecalis CECT7121 stimulus in a dose-dependent manner, inducing apoptosis. The production of ceramide was involved in the latter effect. To undertake in vivo studies, syngeneic BALB ⁄ c mice pre-treated i.p. with Ent. faecalis CECT7121 (2Æ5 · 108 CFU) were challenged i.p. with LBC cells (1Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.Ent. faecalis CECT7121 stimulus in a dose-dependent manner, inducing apoptosis. The production of ceramide was involved in the latter effect. To undertake in vivo studies, syngeneic BALB ⁄ c mice pre-treated i.p. with Ent. faecalis CECT7121 (2Æ5 · 108 CFU) were challenged i.p. with LBC cells (1Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.in vivo studies, syngeneic BALB ⁄ c mice pre-treated i.p. with Ent. faecalis CECT7121 (2Æ5 · 108 CFU) were challenged i.p. with LBC cells (1Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.Ent. faecalis CECT7121 (2Æ5 · 108 CFU) were challenged i.p. with LBC cells (1Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.Æ0 · 106 cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recorded in the control group. A group of surviving mice was re-challenged with LBC cells, and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cell proliferation, high IFNc, IL-12 and IL-10 levels were observed in surviving animals.c, IL-12 and IL-10 levels were observed in surviving animals. Conclusions: Enterococcus faecalis CECT7121 affected multiple factors of the tumour establishment by the following methods: down-regulating the LBC cell proliferation and inducing apoptosis in these cells; and enhancing the immune response that protects animals from lymphoma challenge and re-challenge.Enterococcus faecalis CECT7121 affected multiple factors of the tumour establishment by the following methods: down-regulating the LBC cell proliferation and inducing apoptosis in these cells; and enhancing the immune response that protects animals from lymphoma challenge and re-challenge. Significance and Impact of the Study: This study demonstrate that Ent. faecalisThis study demonstrate that Ent. faecalis CECT7121 has potential as a probiotic that could facilitate the development of novel complements to therapeutic strategies against oncological diseases. Journal of Applied Microbiology ISSN 1364-5072 1234 Journal compilation ª 2010 The Society for Applied Microbiology, Journal of Applied Microbiology 109 (2010) 1234–1243Journal compilation ª 2010 The Society for Applied Microbiology, Journal of Applied Microbiology 109 (2010) 1234–1243 ª 2010 Th2010 Th