CONICET | Buscador de Institutos y Recursos Humanos

We explored the in vitro and in vivo mechanism of antitumor action of the synthetic flavonoid 2´-nitroflavone on LM3 murine mammary adenocarcinoma cells. In vitro assays showed that 2´-nitroflavone increased the population of LM3 hypodiploid cells and produced a typical ladder of DNA fragmentation. Apoptotic cell death was also characterized by the activation of caspase-8, -9 and -3, by an increment in the expression levels of the proapoptotic protein Bax and by the release of cytochrome-c to cytosol. The in vivo effect of 2´-nitroflavone on tumor growth was studied in BALB/c mice injected subcutaneously with LM3 cells. Results showed that tumor volume and weight were significantly reduced at doses of 10 and 40 mg/kg of 20-nitroflavone, respectively. Apoptotic cells were identified by TUNEL assay in tumor slices from mice treated with 10 mg/kg of 2´-nitroflavone. Western blot analysis of tumor lysate supernatants from treated mice revealed an upregulation of the total levels of Bax and Fas receptor. In addition, administration of 40 mg/kg of 2´-nitroflavone to non-tumor-bearing-mice showed no histopathological effects on different organ tissues. This is the first report of the in vivo growth inhibitory effect of 2´-nitroflavone as an apoptotic agent likely useful for mammary adenocarcinoma treatment.